A 127 KDA COMPONENT OF A UV-DAMAGED DNA-BINDING COMPLEX, WHICH IS DEFECTIVE IN SOME XERODERMA-PIGMENTOSUM GROUP-E PATIENTS, IS HOMOLOGOUS TO A SLIME-MOLD PROTEIN

被引:91
作者
TAKAO, M
ABRAMIC, M
MOOS, M
OTRIN, VR
WOOTTON, JC
MCLENIGAN, M
LEVINE, AS
PROTIC, M
机构
[1] NICHHD,DNA REPLICAT REPAIR & MUTAGENESIS SECT,BLDG 6,RM 1A-15,BETHESDA,MD 20892
[2] NATL LIB MED,NATL CTR BIOTECHNOL INFORMAT,BETHESDA,MD 20209
[3] US FDA,DIV CELLULAR & GENE THERAPY,DEV BIOL LAB,BETHESDA,MD 20892
关键词
D O I
10.1093/nar/21.17.4111
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A cDNA which encodes a approximately 127 kDa UV-damaged DNA-binding (UV-DDB) protein with high affinity for (6-4)pyrimidine dimers [Abramic', M., Levine, A.S. & Protic', M., J. Biol. Chem. 266:22493-22500, 1991] has been isolated from a monkey cell cDNA library. The presence of this protein in complexes bound to UV-damaged DNA was confirmed by immunoblotting. The human cognate of the UV-DDB gene was localized to chromosome 11. UV-DDB mRNA was expressed in all human tissues examined, including cells from two patients with xeroderma pigmentosum (group E) that are deficient in UV-DDB activity, which suggests that the binding defect in these cells may reside in a dysfunctional UV-DDB protein. Database searches have revealed significant homology of the UV-DDB protein sequence with partial sequences of yet uncharacterized proteins from Dictyostelium discoideum (44% identity over 529 amino acids) and Oryza sativa (54% identity over 74 residues). According to our results, the UV-DDB polypeptide belongs to a highly conserved, structurally novel family of proteins that may be involved in the early steps of the UV response, e.g., DNA damage recognition.
引用
收藏
页码:4111 / 4118
页数:8
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