IMMUNOASSAY REAGENTS FOR THYROID TESTING .1. SYNTHESIS OF THYROXINE CONJUGATES

被引：21

作者：

ADAMCZYK, M

FINO, L

FISHPAUGH, JR

JOHNSON, DD

MATTINGLY, PG

机构：

[1] Divisional Organic Chemistry Research, Diagnostics Division, Abbott Laboratories, Abbott Park, Illinois 60064-3500, D9NM, Building AP20, One Abbott Park Road

来源：

BIOCONJUGATE CHEMISTRY | 1994年 / 5卷 / 05期

关键词：

D O I：

10.1021/bc00029a013

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Immunoreagents were designed to improve the performance of a commercial fluorescent polarization immunoassay for thyroxine. The thyroxine immunogen was prepared by selective coupling of N-acetyl-L-thyroxine to BSA via an aminocaproic acid spacer arm. The fluorescent tracer was prepared by a multistep reaction sequence which relied on extensive use of orthogonol protecting groups.

引用

页码：459 / 462

页数：4

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