KINETICS OF RELEASE OF 86RUBIDIUM AND HEMOGLOBIN FROM ERYTHROCYTES DAMAGED BY ANTIBODY AND COMPLEMENT

被引：41

作者：

HINGSON, DJ

MASSENGILL, RK

MAYER, MM

机构：

[1] Department of Microbiology, Johns Hopkins University, School of Medicine, Baltimore, MD

来源：

IMMUNOCHEMISTRY | 1969年 / 6卷 / 02期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/0019-2791(69)90166-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sheep erythrocytes carrying 100-200 SAC′ la,4 (rabbit antibody and guinea pig complement) were labelled with 86Rb and treated with varying dilutions of guinea pig C′2 at 30°C. At the peak of SAC't' la, 4,2a generation, C′-EDTA was added and kinetic sampling was begun by means of a simple filtration technique for rapid separation of cells from medium. Release of 86Rb{plus sign, dot below} started after a lag phase of about 0·5-1·0 min, and followed first order kinetics during a prolytic phase lasting 1-15 min, depending on C′2 mulitplicity. Cell lysis then ensued, with first-order release of hemoglobin (Hb). The kinetics of 86Rb{plus sign, dot below} and Hb release form the control populations. A physiological interpreration of the controls is suggested. © 1969.

引用

页码：295 / +

页数：1

共 25 条

[1] LESIONS IN ESCHERICHIA COLI MEMBRANES AFTER ACTION OF ANTIBODY AND COMPLEMENT [J].