PRELIMINARY CRYSTALLOGRAPHIC STUDY OF BLUETONGUE VIRUS CAPSID PROTEIN, VP7

被引：19

作者：

BASAK, AK ^{[1
]}

STUART, DI ^{[1
]}

ROY, P ^{[1
]}

机构：

[1] NERC,INST VIROL & ENVIRONM MICROBIOL,OXFORD OX1 3SR,ENGLAND

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1992年 / 228卷 / 02期

关键词：

VIRUS STRUCTURE; X-RAY DIFFRACTION; CRYSTALLIZATION;

D O I：

10.1016/0022-2836(92)90850-J

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bluetongue virus serotype 10 (BTV-10) VP7, expressed by insect cells infected with the recombinant baculovirus, has been purified and crystallized. Two crystal forms suitable for X-ray analysis have been obtained. Type I crystals belong to space group P6322 with a = b = 95.2 A ̊, c = 181.0 A ̊, α = β = 90 ° γ = 120.0 °, and contain a single subunit in the crystallographic asymmetric unit. They diffract to dmin = 3.0 A ̊. Type II crystals belong to space group P21 with a = 69.4 A ̊, b = 97.1 A ̊, c = 71.4 A ̊, β = 109.0 °, and contain a trimer in the crystallographic asymmetric unit. They diffract to dmin = 2.1 A ̊. These results, together with solution studies, show that the molecule is a trimer. © 1992.

引用

页码：687 / 689

页数：3

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