ROLE OF A DISTAL PROMOTER ELEMENT IN THE S-PHASE CONTROL OF THE HUMAN H1.2 HISTONE GENE-TRANSCRIPTION

被引：12

作者：

EILERS, A ^{[1
]}

BOUTERFA, H ^{[1
]}

TRIEBE, S ^{[1
]}

DOENECKE, D ^{[1
]}

机构：

[1] UNIV GOTTINGEN,INST BIOCHEM & MOLEK ZELLBIOL,MOLEK BIOL ABT,D-37073 GOTTINGEN,GERMANY

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1994年 / 223卷 / 02期

关键词：

D O I：

10.1111/j.1432-1033.1994.tb19026.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The expression of one of the human main type H1 histone genes (termed H1.2) appears to be regulated by several trans-acting factors. Upstream of consensus regulatory regions, such as the TATA-, CCAAT- and H1-box (AAACACA) sequences, a crucial control site is located between nucleotide positions -536 and -412 (relative to the ATG initiation site). Removal of this promoter portion causes in chloramphenicol acetyl transferase reporter gene constructs a loss of the S-phase control function of the H1.2 promoter in HeLa cells. Electrophoretic mobility-shift assay and DNase I footprinting analysis suggest that the H1-box variant AAACAGA is a potential control element within the distal promoter region.

引用

页码：567 / 574

页数：8

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