PRODUCTION OF TAXOL AND RELATED TAXANES IN TAXUS-BREVIFOLIA CELL-CULTURES - EFFECT OF SUGAR

被引：52

作者：

KIM, JH

YUN, JH

HWANG, YS

BYUN, SY

KIM, DI

机构：

[1] INHA UNIV,DEPT BIOL ENGN,INCHON 402751,SOUTH KOREA

[2] AJOU UNIV,DEPT BIOTECHNOL,SUWON 441749,SOUTH KOREA

来源：

BIOTECHNOLOGY LETTERS | 1995年 / 17卷 / 01期

关键词：

D O I：

10.1007/BF00134204

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Suspension cells of Taxus brevifolia were cultured as an alternative source of taxol and related taxanes. The effects of basal medium, sugar type, and sugar concentration on growth and taxane production were investigated. To induce taxol production, modification of medium composition was necessary. Fructose supported the best taxol yield and the use of high concentration of sugar was desirable. The maximum level of taxol obtained after 10 days of culture in an optimized condition was 1.43 mg/L, which was 0.013% as a specific content.

引用

页码：101 / 106

页数：6

共 14 条

[1] SCIENTISTS MOBILIZE TO INCREASE SUPPLY OF ANTICANCER DRUG TAXOL [J].