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COMPARISON OF 5 DIAGNOSTIC METHODS FOR DETECTION OF ROTAVIRUS ANTIGENS IN CALF FECES

被引：45
作者：
ELLENS, DJ
DELEEUW, PW
STRAVER, PJ
VANBALKEN, JAM
机构：
[1] Virology Department, Central Veterinary Institute, Lelystad
来源：
MEDICAL MICROBIOLOGY AND IMMUNOLOGY | 1978年 / 166卷 / 1-4期
关键词：
D O I：
10.1007/BF02121145
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Immunoelectroosmophoresis, complement fixation, immunofluorescence on inoculated cell cultures, electron microscopy, and an enzyme-linked immunosorbent assay were compared for the detection of rotavirus in faecal samples from calves. Rotavirus particles could be detected in 39 out of 98 faecal samples by electron microscopy. Immunofluorescence, complement fixation, and immunoelectroosmophoresis detected rotavirus antigens in 39, 42, and 30 samples, respectively. The enzyme-linked immunosorbent assay demonstrated rotavirus antigens in 49 faecal samples. The assay is not only sensitive but also simple to perform and suitable for large-scale testing. © 1978 Springer-Verlag.
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收藏
页码：157 / 163
页数：7
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