REVERSED-PHASE CHROMATOGRAPHY OF PEPTIDES

The chromatographic behaviour of underivatized peptides on reversed-phase packings has been examined. To study the effects of different amino acids in peptides, simple model substances, such as amino acids and dipeptides, were chosen. The capacity factors of amino acids, l,l-dipeptides and diastereoisomeric dipeptides were measured, and side-effects such as loss by adsorption, double-peak formation and load-retention dependence were studied. Of the four different reversed phases examined, the ODS packings appeared to give both the best selectivity and the best efficiency in the separation of most peptides and diastereoisomers. A marked difference in the retention of basic functions was obtained on two ODS packings of different manufacture. © 1978.