A COMPUTERIZED CALIBRATION OF CIRCULAR DICHROMETER

被引：345

作者：

CASSIM, JY

YANG, JT

机构：

[1] Cardiovascular Research Institute, Department of Biochemistry, University of California San Francisco, Medical Center, San Francisco

来源：

BIOCHEMISTRY | 1969年 / 8卷 / 05期

关键词：

D O I：

10.1021/bi00833a026

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A least-squares parametric curve-fitting method is used to calibrate a circular dichrometer against a standardized spectropolarimeter. An application of this technique is demonstrated by determining the “calibrated” molar circular dichoic absorption, ϵ1-ϵr, of two camphor derivatives in solution. Maximal values of +2.20 for d-10-camphorsulfonic acid in water, + 1.54 for d-camphor in methanol, and +1.69 for dcamphor in dioxane are found at 291, 296, and 300 mμ, respectively. It is concluded that these values may be used as standards for calibration provided some precautions regarding solute and solvent purity are taken. © 1969, American Chemical Society. All rights reserved.

引用

页码：1947 / &

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