QUANTITATIVE-ANALYSIS OF POLYMERASE CHAIN-REACTION (PCR) PRODUCTS USING PRIMERS LABELED WITH BIOTIN AND A FLUORESCENT DYE

被引：60

作者：

LANDGRAF, A ^{[1
]}

RECKMANN, B ^{[1
]}

PINGOUD, A ^{[1
]}

机构：

[1] E MERCK AG,DIAGNOST FORSCHUNG,W-6100 DARMSTADT 1,GERMANY

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 193卷 / 02期

关键词：

D O I：

10.1016/0003-2697(91)90014-K

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

PCR primers covalently labeled with biotin and a fluorescent dye allow immobilization and separation of the products which can be quantitatively analyzed subsequently. The procedure we have developed circumvents electrophoretic separation and radioactive labeling. Exact quantitative analysis of reaction products is feasible during the logarithmic phase of amplification when Taq polymerase is not limiting, as it is during the plateau phase of the reaction. With appropriate standardization the procedure can be used for routine diagnostic purposes. © 1991.

引用

页码：231 / 235

页数：5

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