CAROTENOIDS LOCATED IN HUMAN LYMPHOCYTE SUBPOPULATIONS AND NATURAL-KILLER-CELLS BY RAMAN MICROSPECTROSCOPY

被引：80

作者：

PUPPELS, GJ ^{[1
]}

GARRITSEN, HSP ^{[1
]}

KUMMER, JA ^{[1
]}

GREVE, J ^{[1
]}

机构：

[1] NETHERLANDS RED CROSS BLOOD TRANSFUS SERV,CENT LAB,1066 CX AMSTERDAM,NETHERLANDS

来源：

CYTOMETRY | 1993年 / 14卷 / 03期

关键词：

FLUORESCENCE ACTIVATED CELL SORTING; CANCER; GALL BODY; GOLGI COMPLEX; FLUORESCENCE MICROSCOPY;

D O I：

10.1002/cyto.990140303

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The presence and subcellular location of carotenoids in human lymphocyte subpopulations (CD4+, CD8+, T-cell receptor-gammadelta+, and CD19+) and natural killer cells (CD16+) were studied by means of Raman microspectroscopy. In CD4+ lymphocytes a high concentration (approximately 10(-3) M) of carotenoids was found in the Gall body. In CD8+ lymphocytes, T-cell-receptor-gammadelta+ lymphocytes and in natural killer cells carotenoids appeared to be concentrated (approximately 10(-4) M) in the Golgi complex. The concentration of carotenoids in CD19+ lymphocytes was found to be below the present detection limit, which is approximately 10(-6) to 10(-5) M. The results provide new possibilities to investigate the mechanism(s) behind the suggested protective role of carotenoids against the development of cancers.

引用

页码：251 / 256

页数：6

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