STEREOSELECTIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF KETOPROFEN, IBUPROFEN AND FENOPROFEN IN PLASMA USING A CHIRAL ALPHA-1-ACID GLYCOPROTEIN COLUMN

被引：78

作者：

MENZELSOGLOWEK, S

GEISSLINGER, G

BRUNE, K

机构：

[1] Department of Pharmacology and Toxicology, University of Erlangen-Nürnberg, D-8520 Erlangen

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1990年 / 532卷 / 02期

关键词：

D O I：

10.1016/S0378-4347(00)83780-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The effect of mobile phase composition, pH and temperature on the chiral resolution and retention of some 2-arylpropionic acids using the chiral α1-acid glycoprotein column EnantioPac® is described. Furthermore, a direct stereoselective high-performance liquid chromatographic assay to determine the enantiomers of ketoprofen, ibuprofen and fenoprofen in plasma is presented. Detection was at 260, 220 and 220 nm for ketoprofen, ibuprofen and fenoprofen, respectively. The limit of detection was 0.1 μg/ml for the enantiomers of ketoprofen and ibuprofen, and 0.25 μg/ml for the enantiomers of fenoprofen. The method was demonstrated to be applicable for stereoselective pharmacokinetic studies of ketoprofen, ibuprofen and fenoprofen after administration under clinical conditions. © 1990.

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页码：295 / 303

页数：9

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