THE EFFECTS OF MICROTUBULE AND MICROFILAMENT DISRUPTING AGENTS ON CYTOSKELETAL ARRAYS AND WALL DEPOSITION IN DEVELOPING COTTON FIBERS

被引:142
作者
SEAGULL, RW
机构
[1] Southern Regional Research Center, USDA/ARS, New Orleans, 70179, LA
关键词
MICROTUBULES; MICROFILAMENTS; WALL MICROFIBRILS; COTTON FIBER; CYTOSKELETAL DISRUPTION;
D O I
10.1007/BF01326634
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The effects of various cytoskeletal disrupting agents (chlochicine, oryzalin, trifluralin, taxol, cytochalasins B and D) on microtubules, microfilaments and wall microfibril deposition were monitored in developing cotton fibers, using immunocytochemical and fluorescence techniques. Treatment with 10(-4) M colchicine, 10(-6) M trifluralin or 10(-6) M oryzalin resulted in a reduction in the number of microtubules, however, the "drug-stable" microtubules still appear to influence wall deposition. Treatment with 10(-5) M taxol increased the numbers of microtubules present within 15 minutes of application. New microtubules were aligned parallel to the existing ones, however, some evidence of random arrays was observed. Microtubules stabilized with taxol appeared to function in wall organization but do not undergo normal re-orientations during development. Microtubule disrupting agent had no detectable affect on the microfilament population. Exposure to either 4 X 10(-5) M cytochalasin B or 2 X 10(-6) M cytochalasin D resulted in a disruption of microfilaments and a re-organization of microtubule arrays. Treatment with either cytochalasin caused a premature shift in the orientation of microtubules in young fibers, whereas in older fibers the microtubule arrays became randomly organized. These observations indicate that microtubule populations during interphase are heterogeneous, differing at least in their susceptibility to disruption by depolymerizing agents. Changes in microtubule orientation (induced by cytochalasin) indicate that microfilaments may be involved in regulating microtubule orientation during development.
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页码:44 / 59
页数:16
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