REMOVAL OF THYMINE-CONTAINING PYRIMIDINE DIMERS FROM UV LIGHT-IRRADIATED DNA BY S1 ENDONUCLEASE

被引：12

作者：

HEFLICH, RH ^{[1
]}

MAHONEYLEO, E ^{[1
]}

MAHER, VM ^{[1
]}

MCCORMICK, JJ ^{[1
]}

机构：

[1] MICHIGAN STATE UNIV,DEPT BIOCHEM,CARCINOGENESIS LAB,E LANSING,MI 48824

来源：

PHOTOCHEMISTRY AND PHOTOBIOLOGY | 1979年 / 30卷 / 02期

关键词：

D O I：

10.1111/j.1751-1097.1979.tb07142.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Abstract— S1 endonuclease was shown to remove thymine‐containing pyrimidine dimers from UV‐irradiated human DNA, although efficient removal could be demonstated only by using long digestion times, relatively high enzyme concentrations, and irradiation sufficient to yield dimer substitutions in DNA of 1 per 1W300 (dimers/base pair). Neutral and alkaline sucrose gradient analysis of strand break induction by S, of UV‐irradiated DNA suggests that recognition of the dimer by S, is the limiting factor in its removal and dimer removal usually results from attack on the dimer containing DNA strand without the induction of a double‐strand break. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：247 / 250

页数：4

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