MEASUREMENT OF RAPAMYCIN IN WHOLE-BLOOD USING REVERSE-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：59

作者：

YATSCOFF, RW ^{[1
]}

FARACI, C ^{[1
]}

BOLINGBROKE, P ^{[1
]}

机构：

[1] UNIV ALBERTA,DEPT PATHOL,EDMONTON T6G 2E1,ALBERTA,CANADA

来源：

THERAPEUTIC DRUG MONITORING | 1992年 / 14卷 / 02期

关键词：

RAPAMYCIN; MEASUREMENT; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY;

D O I：

10.1097/00007691-199204000-00011

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Rapamycin (RAPA) is an immunosuppressant with a potency similar to FK 506 and up to 100-fold greater than cyclosporine. To date, no data have been reported on the pharmacokinetics and disposition of the drug, primarily due to a lack of suitable methods for its analysis. We describe here an evaluation of a reverse-phase high-performance liquid chromatographic (HPLC) method for analysis of the drug in whole blood. The method showed excellent analytical recovery of the drug, close to 100%. The sensitivity method was found to be 1.0-mu-g/L with between-run coefficients of variation of 14.4% and 9.8% at 10 and 50 mg/L. The stability of RAPA was investigated with this method in whole-blood specimens. It was found that the drug was stable for 30 days at 4-degrees-C and for 55 days at -40-degrees-C.

引用

页码：138 / 141

页数：4

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