ADMINISTRATION OF AN ADENOVIRUS CONTAINING THE HUMAN CFTR CDNA TO THE RESPIRATORY-TRACT OF INDIVIDUALS WITH CYSTIC-FIBROSIS

被引：730

作者：

CRYSTAL, RG ^{[1
]}

MCELVANEY, NG ^{[1
]}

ROSENFELD, MA ^{[1
]}

CHU, CS ^{[1
]}

MASTRANGELI, A ^{[1
]}

HAY, JG ^{[1
]}

BRODY, SL ^{[1
]}

JAFFE, HA ^{[1
]}

EISSA, NT ^{[1
]}

DANEL, C ^{[1
]}

机构：

[1] NHLBI,PULM BRANCH,BETHESDA,MD 20892

来源：

NATURE GENETICS | 1994年 / 8卷 / 01期

关键词：

D O I：

10.1038/ng0994-42

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have administered a recombinant adenovirus vector (AdCFTR) containing the normal human CFTR cDNA to the nasal and bronchial epithelium of four individuals with cystic fibrosis (CF). We show that this vector can express the CFTR cDNA in the CF respiratory epithelium in vivo. With doses up to 2 x 10(9) pfu, there was no recombination/ complementation or shedding of the vector or rise of neutralizing antibody titres. At 2 x 10(9) pfu, a transient systemic and pulmonary syndrome was observed, possibly mediated by interleukin-6. Follow-up at 6-12 months demonstrated no long term adverse effects. Thus, it is feasible to use an adenovirus vector to transfer and express the CFTR cDNA in the respiratory epithelium of individuals with CF. Correction of the CF phenotype of the airway epithelium might be achieved with this strategy.

引用

页码：42 / 51

页数：10

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