SURVEY OF MAJOR GENOTYPES AND SUBTYPES OF HEPATITIS-C VIRUS USING RFLP OF SEQUENCES AMPLIFIED FROM THE 5' NONCODING REGION

被引：380

作者：

DAVIDSON, F

SIMMONDS, P

FERGUSON, JC

JARVIS, LM

DOW, BC

FOLLETT, EAC

SEED, CRG

KRUSIUS, T

LIN, C

MEDGYESI, GA

KIYOKAWA, H

OLIM, G

DURAISAMY, G

CUYPERS, T

SAEED, AA

TEO, D

CONRADIE, J

KEW, MC

LIN, M

NUCHAPRAYOON, C

NDIMBIE, OK

YAP, PL

机构：

[1] ROYAL EDINBURGH & ASSOCIATED HOSP, EDINBURGH & SE SCOTLAND BLOOD TRANSFUS SERV, EDINBURGH EH3 9HB, MIDLOTHIAN, SCOTLAND

[2] UNIV EDINBURGH, DEPT MED MICROBIOL, EDINBURGH EH8 9AG, MIDLOTHIAN, SCOTLAND

[3] RUCHILL HOSP, SCOTTISH NATL BLOOD TRANSFUS SERV MICROBIOL REFER, GLASGOW, LANARK, SCOTLAND

[4] AUSTRALIAN RED CROSS SOC, BLOOD TRANSFUS SERV, PERTH, WA, AUSTRALIA

[5] FINNISH RED CROSS & BLOOD TRANSFUS SERV, SF-00310 HELSINKI, FINLAND

[6] HONG KONG RED CROSS, BLOOD TRANSFUS SERV, YAUMATEI, HONG KONG

[7] NATL INST HAEMATOL BLOOD TRANSFUS & IMMUNOL, H-1113 BUDAPEST, HUNGARY

[8] FUKUOKA RED CROSS, CTR BLOOD TRANSFUS, FUKUOKA 818, JAPAN

[9] MACAU BLOOD TRANSFUS CTR, Macau, Macao, PEOPLES R CHINA

[10] HOSP KUALA LUMPUR, NATL BLOOD SERV CTR, 50586 KUALA LUMPUR, MALAYSIA

[11] NETHERLANDS RED CROSS, BLOOD TRANSFUS SERV,CENT LAB, 1066 CX AMSTERDAM, NETHERLANDS

[12] RIYADH ARMED FORCES HOSP, RIYADH 11159, SAUDI ARABIA

[13] NATL BLOOD CTR, SINGAPORE BLOOD TRANSFUS SERV, SINGAPORE 0316, SINGAPORE

[14] NATAL BLOOD TRANSFUS SERV, NATAL INST IMMUNOL, DURBAN 4000, SOUTH AFRICA

[15] UNIV WITWATERSRAND, SCH MED, JOHANNESBURG, SOUTH AFRICA

[16] MACKAY MEM HOSP, NATL HLTH RES INST,TRANSFUS MED LAB, TAIPEI, TAIWAN

[17] THAI RED CROSS SOC, NATL BLOOD CTR, BANGKOK 10330, THAILAND

[18] CENT BLOOD BANK PITTSBURGH, PITTSBURGH, PA USA

来源：

JOURNAL OF GENERAL VIROLOGY | 1995年 / 76卷

关键词：

D O I：

10.1099/0022-1317-76-5-1197

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A method is described for identifying different genotypes of hepatitis C virus (HCV) by restriction endonuclease cleavage of sequences amplified by PCR from the 5' non-coding region. Using the enzymes HaeIII-RsaI and HinfI-MvaI, followed by cleavage with BstU1 or ScrFI, it was possible to identify and distinguish HCV genotypes 1a, 1b, 2a, 2b, 3a, 3b, 4, 5 and 6. The method was used to investigate the prevalence of these genotypes in 723 blood donors in 15 countries, the largest survey to date, and one which covered a wide range of geographical regions (Europe, America, Africa and Asia). These results, combined with a review of the existing literature, indicate the existence of several distinct regional patterns of HCV genotype distribution, and provide the framework for future detailed epidemiological investigations of HCV transmission.

引用

页码：1197 / 1204

页数：8