SEPARATION OF FLAVONOID GLUCOSIDES FROM THEIR GALACTOSIDIC ANALOGS BY THIN-LAYER CHROMATOGRAPHY

Particular problems in flavonoid analysis such as the separation of mixtures of glucosyl and galactosyl analogues appears to be solvable by means of thin-layer chromatography in classical flat chambers. Each of the nine pairs of such analogues tested, including flavonol 3-O-glycosides (three pairs), flavonol 3-O-diglycosides (two pairs), mono-C-glycosylflavones (one pair), di-C-glycosylflavones (one pair) and C,O-glycosylflavones (two pairs) could be resolved into individual components in at least one of the nine chromatographic systems described. The method is recommended for homogeneity control of C-glycosylflavones and any flavonoid which releases both glucose and galactose on hydrolysis.