USE OF N-ACETYLGLUCOSAMINYLTRANSFERASE-I AND N-ACETYLGLUCOSAMINYLTRANSFERASE-II IN THE PREPARATIVE SYNTHESIS OF OLIGOSACCHARIDES

被引：32

作者：

KAUR, KJ ^{[1
]}

ALTON, G ^{[1
]}

HINDSGAUL, O ^{[1
]}

机构：

[1] UNIV ALBERTA,DEPT CHEM,EDMONTON T6G 2G2,ALBERTA,CANADA

来源：

CARBOHYDRATE RESEARCH | 1991年 / 210卷

关键词：

D O I：

10.1016/0008-6215(91)80118-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

8-Methoxycarbonyloctyl 3,6-di-O-(alpha-D-mannopyranosyl)-beta-D-mannopyranoside (1) has been synthesised chemically. Compound 1 is a substrate for N-acetylglucosaminyltransferase-I (GlcNAcT-I), which transfers a beta-D-GlcpNAc residue from UDP-GlcpNAc to position 2 of the alpha-Man-(1 --> 3) unit to produce 2. In turn, the tetrasaccharide 2 is an acceptor for GlcNAcT-II which, in the presence of UDP-GlcpNAc, converts 2 into 8-methoxycarbonyloctyl 3,6-di-O-[2-O-(2-acetamido-2-deoxy-beta-D-glucopyranosyl)-alpha-D-mannopyranosyl]-beta-D-mannopyranoside (3). These conversions were carried out on a 50-100 mg scale using enzyme preparations obtained from rabbit liver in a single step by affinity chromatography.

引用

页码：145 / 153

页数：9

共 25 条

[1] SYNTHESIS OF A TRI-SACCHARIDE, A PENTA-SACCHARIDE, AND A HEPTA-SACCHARIDE CONTAINING TERMINAL N-ACETYL-BETA-D-LACTOSAMINYL RESIDUES, PART OF THE COMPLEX-TYPE CARBOHYDRATE MOIETY OF GLYCOPROTEINS [J].