P-32 POSTLABELING ANALYSIS OF ADDUCTS FORMED FROM 1-PHENYLAZO-2-HYDROXYNAPHTHALENE (SUDAN I, SOLVENT YELLOW 14) WITH DNA AND HOMOPOLYDEOXYRIBONUCLEOTIDES

被引：21

作者：

STIBOROVA, M ^{[1
]}

FREI, E ^{[1
]}

SCHMEISER, HH ^{[1
]}

WIESSLER, M ^{[1
]}

机构：

[1] GERMAN CANC RES CTR, INST TOXICOL & CHEMOTHERAPY, W-6900 HEIDELBERG 1, GERMANY

来源：

CARCINOGENESIS | 1992年 / 13卷 / 07期

关键词：

D O I：

10.1093/carcin/13.7.1221

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A P-32-postlabeling assay was employed for detection and quantitation of DNA adducts formed with carcinogenic 1-phenylazo-2-hydroxynaphthalene (Sudan I, Solvent Yellow 14) activated by a peroxidase system. Enrichment of adducts by digestion with nuclease P1 or by extraction into n-butanol prior to-P-32-labeling was used. Both enrichment procedures exhibited comparable results for recovery of individual DNA adduct spots. Co-chromatographic analyses of adduct spots obtained by reaction with DNA and homopolydeoxyribonucleotides showed that four out of the eight major Sudan I - DNA adducts were formed by reaction of activated Sudan I with deoxyadenosine or deoxyguanosine in DNA. The accuracy of quantitation of adducts by P-32-postlabeling procedure is discussed.

引用

页码：1221 / 1225

页数：5

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