PROPERTIES OF POLY(3-HYDROXYBUTYRATE) DEPOLYMERASE FROM A MARINE BACTERIUM, ALCALIGENES-FAECALIS AE122

被引：65

作者：

KITA, K ^{[1
]}

ISHIMARU, K ^{[1
]}

TERAOKA, M ^{[1
]}

YANASE, H ^{[1
]}

KATO, N ^{[1
]}

机构：

[1] TOTTORI UNIV,DEPT BIOTECHNOL,TOTTORI 680,JAPAN

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1995年 / 61卷 / 05期

关键词：

D O I：

10.1128/AEM.61.5.1727-1730.1995

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Alcaligenes faecalis AE122 that used poly(3-hydroxybutyrate) (PHB) as a sole source of carbon was newly isolated from a coastal seawater sample. The strain required seawater for growth on PHB as well as in a nutrient broth, in which seawater could be replaced by an appropriate concentration of NaCl. PHB depolymerase was purified to homogeneity from the culture supernatant of A. faecalis AE122 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The enzyme consisted of a monomer subunit with a molecular mass of 95.5 kDa. The N-terminal amino acid sequence was GAWQNNLAGGFNKV. The dimeric and trimeric esters of 3-hydroxybutyrate were the main hydrolysis products of the purified enzyme. The enzyme was most active at pH 9.0 and 55 degrees C and was inhibited by phenylmethylsulfonyl fluoride. Several cations in seawater greatly enhanced the enzyme activity.

引用

页码：1727 / 1730

页数：4

共 20 条

[1] OCCURRENCE, METABOLISM, METABOLIC ROLE, AND INDUSTRIAL USES OF BACTERIAL POLYHYDROXYALKANOATES [J].