BISACK ANALYSIS OF THE PHYTOHEMAGGLUTININ-INDUCED PROLIFERATION OF HUMAN PERIPHERAL LYMPHOCYTES

被引：31

作者：

TICE, R ^{[1
]}

THORNE, P ^{[1
]}

SCHNEIDER, EL ^{[1
]}

机构：

[1] NIA,GERONTOL RES CTR,BALTIMORE,MD

来源：

CELL AND TISSUE KINETICS | 1979年 / 12卷 / 01期

关键词：

D O I：

10.1111/j.1365-2184.1979.tb00108.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The rate of stimulation as well as subsequent cell cycle duration was examined in phytohaemagglutinin‐stimulated human peripheral lymphocytes grown in vitro in the presence of non‐inhibitory concentrations of bromodeoxyuridine. After incorporation of this heavy atom analogue of thymidine into replicating cellular DNA, it was possible to identify unequivocally metaphase cells which had replicated for one, two and three or more cell cycles. Utilizing this technique, distribution curves were obtained for the appearance of metaphase cells in successive generations, were analysed by a computer simulation model, and the rate of stimulation (4.5% per hr of the remaining unstimulated population) and cell cycle duration (12·3 hr) were determined. The results were compared with those obtained by autoradiography and the possible relationship to the ‘transition probability’ model for cellular proliferation is discussed. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：1 / 9

页数：9

共 31 条

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