Eye movements associated with eye closure were studied in 36 normal adults, and they were recorded by DC amplifiers of ENG. (1) Eyes moved 64 ± 17° upward and were adducted 15 ± 7° when eyes were closed. (2) The vertical eye position was held upwards in 33 persons and slightly turned down in 3 persons. (3) The superior rectus and inferior oblique muscles worked together during eye closure.