INFLUENCE OF THE POLY(A) TAIL AND PUTATIVE POLYADENYLATION SIGNAL ON THE INFECTIVITY OF WHITE CLOVER MOSAIC POTEXVIRUS

被引:45
作者
GUILFORD, PJ
BECK, DL
FORSTER, RLS
机构
[1] DSIR Plant Protection, Mt. Albert Research Centre, Auckland, Private Bag
关键词
D O I
10.1016/0042-6822(91)90648-U
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A series of mutations has been constructed in a cDNA clone of white clover mosaic virus (WCIMV) which decreases the poly(A) tail length of run-off transcripts from 74 to 27, 10 or zero 3′-terminal (A) residues. Although transcripts with short poly(A) tails were less infectious than wild-type RNA, complete removal of the (A) tail did not abolish infectivity. Addition of nonviral nucleotides to the 3′ terminus of transcripts with no 3′-terminal (A) residues eliminated infectivity. Heterogenous-length poly(A) tails, indistinguishable from the wild type, were synthesized de novo in plants inoculated with transcripts with no 3′-terminal (A) residues, demonstrating the presence of a poly(A) polymerase activity in WCIMV-infected tissue. Mutation of a putative polyadenylation motif found in the 3′ noncoding region of WCIMV decreased the efficiency of polyadenylation of the progeny of transcripts with 10 3′-terminal (A) residues. The same mutation in transcripts with no 3′-terminal (A) residues abolished infectivity. © 1991.
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页码:61 / 67
页数:7
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