SODIUM AND CALCIUM LOCALIZATION IN CELLS AND TISSUES BY PRECIPITATION WITH ANTIMONATE - QUANTITATIVE STUDY

被引：48

作者：

VANIREN, F ^{[1
]}

VANESSENJOOLEN, L ^{[1
]}

VANDERDUYNSCHOUTEN, P ^{[1
]}

BOERSVANDERSLUIJS, P ^{[1
]}

DEBRUIJN, WC ^{[1
]}

机构：

[1] RIJKSUNIV, ANALYT ELECTRON MICROSCOPY, ELECTRONENMICROSCOPIE LAB, NL-2333 AA LEIDEN, NETHERLANDS

来源：

HISTOCHEMISTRY | 1979年 / 63卷 / 03期

关键词：

D O I：

10.1007/BF00490056

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Komnick's antimonate technique, which was devised to localize Na+ in cells and tissues, was studied quantitatively. Some modifications, as well as its application to Ca2+ localization, were also investigated. We combined measurements of Na+ and Ca2+ retention in plant roots during the various procedures, electron microscopy, autoradiography, and semiquantitative X-ray microanalysis. We were able to show that (at least in barley roots) antimonate does not precipitate at all with Na+, irrespective of the Na+ content of the tissue or the method of antimonate application. (Even during precipitative freeze dissolution or after freeze drying, no Na+ is precipitated.) By means of Komnick's antimonate technique Ca2+ is trapped within the tissue, but only after serious dislocation. Perspectives for reliable localization of diffusible ions in cells and tissues, by precipitation simultaneously with conventional fixations, are bad. © 1979 Springer-Verlag.

引用

页码：273 / 294

页数：22

共 72 条

[1] LOCALIZATION OF PYROANTIMONATE-PRECIPITABLE CATION AND SURFACE COAT ANIONIC BINDING-SITES IN DEVELOPING ERYTHROCYTIC CELLS AND MACROPHAGES IN NORMAL HUMAN BONE-MARROW [J].