THE FUNCTION OF DNA-POLYMERASES IN DNA-REPAIR SYNTHESIS OF ULTRAVIOLET-IRRADIATED HUMAN FIBROBLASTS

被引：31

作者：

POPANDA, O ^{[1
]}

THIELMANN, HW ^{[1
]}

机构：

[1] GERMAN CANC RES CTR,INST BIOCHEM,NEUENHEIMER FELD 280,W-6900 HEIDELBERG,GERMANY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1129卷 / 02期

关键词：

DNA POLYMERASE; DNA REPAIR SYNTHESIS; ULTRAVIOLET IRRADIATION; (HUMAN FIBROBLAST);

D O I：

10.1016/0167-4781(92)90480-N

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ultraviolet-induced DNA repair synthesis was measured in saponin-permeabilized normal human fibroblasts by the incorporation of [alpha-P-32]dTMP into DNA. The involvement of DNA polymerases alpha, beta, delta, and epsilon in excision repair of pyrimidine dimers was examined using specific inhibitors. Dose-response curves resulting from experiments with up to 12 different inhibitor concentrations were analyzed by linear regression. Inhibitor concentrations at which repair activity was reduced to 50% were calculated. The following K50 values were found: aphidicolin, 0.2-mu-M; ddTTP, 12.5-mu-M; butylphenyl-dGTP, 7.6-mu-M; butylanilino-dATP, 6.0-mu-M. Comparison of K50 values with in vitro K(i) values of DNA polymerases revealed that in permeabilized human fibroblasts reparative DNA synthesis is catalyzed by DNA polymerase delta and by DNA polymerase epsilon.

引用

页码：155 / 160

页数：6

共 32 条

[1] PROPERTIES OF DNA-POLYMERASES DELTA AND EPSILON, AND THEIR ROLES IN EUKARYOTIC DNA-REPLICATION [J].

BAMBARA, RA ;

JESSEE, CB .

BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1088 (01) :11-24

[2] APHIDICOLIN INHIBITS EUKARYOTIC DNA-REPLICATION AND REPAIR - IMPLICATIONS FOR INVOLVEMENT OF DNA POLYMERASE-ALPHA IN BOTH PROCESSES [J].

BERGER, NA ;

KUROHARA, KK ;

PETZOLD, SJ ;

SIKORSKI, GW .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1979, 89 (01) :218-225

[3] REVISED NOMENCLATURE FOR EUKARYOTIC DNA-POLYMERASES [J].

BURGERS, PMJ ;

BAMBARA, RA ;

CAMPBELL, JL ;

CHANG, LMS ;

DOWNEY, KM ;

HUBSCHER, U ;

LEE, MYWT ;

LINN, SM ;

SO, AG ;

SPADARI, S .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1990, 191 (03) :617-618

[4] INCREASED NUCLEAR CYCLIN/PCNA ANTIGEN STAINING OF NON S-PHASE TRANSFORMED HUMAN AMNION CELLS ENGAGED IN NUCLEOTIDE EXCISION DNA-REPAIR [J].

CELIS, JE ;

MADSEN, P .

FEBS LETTERS, 1986, 209 (02) :277-283

[5] STRUCTURE OF REPAIRED SITES IN HUMAN DNA SYNTHESIZED IN THE PRESENCE OF INHIBITORS OF DNA POLYMERASE-ALPHA AND POLYMERASE-BETA IN HUMAN-FIBROBLASTS [J].

CLEAVER, JE .

BIOCHIMICA ET BIOPHYSICA ACTA, 1983, 739 (03) :301-311

[6] PURIFICATION AND CHARACTERIZATION OF 2 NEW HIGH-MOLECULAR-WEIGHT FORMS OF DNA POLYMERASE-DELTA [J].

CRUTE, JJ ;

WAHL, AF ;

BAMBARA, RA .

BIOCHEMISTRY, 1986, 25 (01) :26-36

[7] ANALYSIS OF BUTYLPHENYL-GUANINE, BUTYLPHENYL-DEOXYGUANOSINE, AND BUTYLPHENYL-DEOXYGUANOSINE TRIPHOSPHATE INHIBITION OF DNA-REPLICATION AND ULTRAVIOLET-INDUCED DNA-REPAIR SYNTHESIS USING PERMEABLE HUMAN-FIBROBLASTS [J].

DRESLER, SL ;

FRATTINI, MG .

BIOCHEMICAL PHARMACOLOGY, 1988, 37 (06) :1033-1037

[8] DNA-REPLICATION AND UV-INDUCED DNA-REPAIR SYNTHESIS IN HUMAN-FIBROBLASTS ARE MUCH LESS SENSITIVE THAN DNA POLYMERASE-ALPHA TO INHIBITION BY BUTYLPHENYL-DEOXYGUANOSINE TRIPHOSPHATE [J].

DRESLER, SL ;

FRATTINI, MG .

NUCLEIC ACIDS RESEARCH, 1986, 14 (17) :7093-7102

[9] INVOLVEMENT OF DNA POLYMERASE-DELTA IN DNA-REPAIR SYNTHESIS IN HUMAN-FIBROBLASTS AT LATE TIMES AFTER ULTRAVIOLET-IRRADIATION [J].

DRESLER, SL ;

GOWANS, BJ ;

ROBINSONHILL, RM ;

HUNTING, DJ .

BIOCHEMISTRY, 1988, 27 (17) :6379-6383

[10]

DRESLER SL, 1983, J BIOL CHEM, V258, P9990

← 1 2 3 4 →