RHIZOBIUM-FREDII AND RHIZOBIUM-MELILOTI PRODUCE 3-DEOXY-D-MANNO-2-OCTULOSONIC ACID-CONTAINING POLYSACCHARIDES THAT ARE STRUCTURALLY ANALOGOUS TO GROUP-II K-ANTIGENS (CAPSULAR POLYSACCHARIDES) FOUND IN ESCHERICHIA-COLI

被引:127
作者
REUHS, BL
CARLSON, RW
KIM, JS
机构
[1] Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602-4712
关键词
D O I
10.1128/jb.175.11.3570-3580.1993
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The polysaccharide components from cultured cells of Rhizobium fredii USDA205 and Rhizobium meliloti AK631 were extracted with hot phenol-water and separated by repetitive gel filtration chromatography. Polyacrylamide gel electrophoresis, nuclear magnetic resonance spectrometry, and gas chromatography analyses showed that both of these bacterial species produce unique polysaccharides that contain a high proportion of 3-deoxy-D-manno-2-octulosonic acid (Kdo). These polysaccharides, which constituted a major portion of the extracted carbohydrate, are not excreted into the growth media (i.e., they are not extracellular polysaccharides) and are structurally distinct from the lipopolysaccharides. The primary structure of the preponderant polysaccharide from R. fredii USDA205 was determined by high-performance anion-exchange liquid chromatography, nuclear magnetic resonance spectrometry, fast atom bombardment-mass spectrometry, and gas chromatography-mass spectrometry; it consists of repeating units of [-->3)-alpha-D-Galp-(1-->5)-beta-D-Kdop-(2-->]n. This molecule is structurally analogous to the constituents of one subgroup of K antigens (capsular polysaccharides) produced by Escherichia coli. Polysaccharides of this type have not previously been identified as components of rhizobial cells. The Kdo-containing polysaccharide from R. meliloti, which has not been completely characterized, appears to be structurally related to that of R. fredii.
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页码:3570 / 3580
页数:11
相关论文
共 44 条
[1]  
BOCK K, 1982, ANNU REP NMR SPECTRO, V13, P1
[2]   A MUTATION THAT BLOCKS EXOPOLYSACCHARIDE SYNTHESIS PREVENTS NODULATION OF PEAS BY RHIZOBIUM-LEGUMINOSARUM BUT NOT OF BEANS BY R-PHASEOLI AND IS CORRECTED BY CLONED DNA FROM RHIZOBIUM OR THE PHYTOPATHOGEN XANTHOMONAS [J].
BORTHAKUR, D ;
BARBER, CE ;
LAMB, JW ;
DANIELS, MJ ;
DOWNIE, JA ;
JOHNSTON, AWB .
MOLECULAR & GENERAL GENETICS, 1986, 203 (02) :320-323
[3]   COMMON LOCI FOR AGROBACTERIUM-TUMEFACIENS AND RHIZOBIUM-MELILOTI EXOPOLYSACCHARIDE SYNTHESIS AND THEIR ROLES IN PLANT INTERACTIONS [J].
CANGELOSI, GA ;
HUNG, L ;
PUVANESARAJAH, V ;
STACEY, G ;
OZGA, DA ;
LEIGH, JA ;
NESTER, EW .
JOURNAL OF BACTERIOLOGY, 1987, 169 (05) :2086-2091
[4]  
Carlson R. W., 1992, Plant biotechnology and development., P33
[6]   HOST-SYMBIONT INTERACTIONS .3. PURIFICATION AND PARTIAL CHARACTERIZATION OF RHIZOBIUM LIPOPOLYSACCHARIDES [J].
CARLSON, RW ;
SANDERS, RE ;
NAPOLI, C ;
ALBERSHEIM, P .
PLANT PHYSIOLOGY, 1978, 62 (06) :912-917
[7]   CHARACTERIZATION OF THE LIPOPOLYSACCHARIDE FROM A RHIZOBIUM-PHASEOLI MUTANT THAT IS DEFECTIVE IN INFECTION THREAD DEVELOPMENT [J].
CARLSON, RW ;
KALEMBASA, S ;
TUROWSKI, D ;
PACHORI, P ;
NOEL, KD .
JOURNAL OF BACTERIOLOGY, 1987, 169 (11) :4923-4928
[8]  
Carlson RW, 1982, NITROGEN FIXATION, V2
[9]   ISOLATION AND CHARACTERIZATION OF THE LIPOPOLYSACCHARIDES FROM BRADYRHIZOBIUM-JAPONICUM [J].
CARRION, M ;
BHAT, UR ;
REUHS, B ;
CARLSON, RW .
JOURNAL OF BACTERIOLOGY, 1990, 172 (04) :1725-1731
[10]   ALTERATION OF THE EFFECTIVE NODULATION PROPERTIES OF A FASTGROWING BROAD HOST RANGE RHIZOBIUM DUE TO CHANGES IN EXOPOLYSACCHARIDE SYNTHESIS [J].
CHEN, H ;
BATLEY, M ;
REDMOND, J ;
ROLFE, BG .
JOURNAL OF PLANT PHYSIOLOGY, 1985, 120 (04) :331-349