ORNITHINE DECARBOXYLASE FROM CRITHIDIA-FASCICULATA IS METABOLICALLY UNSTABLE AND RESISTANT TO POLYAMINE DOWN-REGULATION

被引:21
作者
CERIANI, C
GONZALEZ, NS
ALGRANATI, ID
机构
[1] UNIV BUENOS AIRES,FAC CIENCIAS EXACTAS & NAT,INST INVEST BIOQUIM,FDN CAMPOMAR,A MACHADO 151,RA-1405 BUENOS AIRES,ARGENTINA
[2] CONSEJO NACL INVEST CIENT & TECN,BUENOS AIRES,ARGENTINA
关键词
ORNITHINE DECARBOXYLASE; ENZYME TURNOVER; POLYAMINE-DEPENDENT REGULATION; CRITHIDIA-FASCICULATA;
D O I
10.1016/0014-5793(92)80253-D
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Ornithine decarboxylase (ODC) of Crithidia fasciculata extracts shows maximal activity during exponential growth of the parasite and decreases markedly in the stationary phase. The inhibition of protein synthesis by cycloheximide evoked a rapid loss of enzyme activity with a half-life of about 30 min. Upon removal of DFMO from Crithidia cultures treated with the drug for 24 h, the ODC activity increased at the same rate as total protein synthesis. The addition of putrescine at high concentrations to parasites cultivated in a synthetic medium showed that Crithidia ODC levels were not reduced by polyamines.
引用
收藏
页码:261 / 264
页数:4
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