EXPRESSION OF CELL-SURFACE MARKERS AND MYELIN PROTEINS IN CULTURED OLIGODENDROCYTES FROM NEONATAL BRAIN OF RAT AND MOUSE - A COMPARATIVE-STUDY

被引：38

作者：

DUCHALA, CS ^{[1
]}

ASOTRA, K ^{[1
]}

MACKLIN, WB ^{[1
]}

机构：

[1] UNIV CALIF LOS ANGELES,MED CTR,MENTAL RETARDAT RES CTR,DEPT PSYCHIAT & BIOBEHAV SCI,LOS ANGELES,CA 90024

来源：

DEVELOPMENTAL NEUROSCIENCE | 1995年 / 17卷 / 02期

关键词：

MIXED GLIAL CULTURES; MYELIN PROTEINS; OLIGODENDROCYTES; MIXED CULTURES; PROGENITORS;

D O I：

10.1159/000111276

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Dissociated brain cell cultures are a useful model for investigating development and differentiation of oligodendrocytes in vitro. The current studies compare the developmental patterns of expression for oligodendrocyte lineage/myelin markers in both primary and secondary oligodendrocyte cultures derived from mouse and rat neonates. The rat and mouse dissociated brain cell cultures express the same myelin-specific antigens, but mouse oligodendrocytes produce a larger and more elaborate sheet-like membrane than rat oligodendrocytes, and some of the myelin markers (O4, GC, and MBP) show more intense membrane staining in mouse cultures. GD(3) appears to be a good oligodendrocyte marker for rat cells, but it is nonspecific in mouse cells. There are fewer oligodendrocytes in mouse cultures, and they appear to require a longer differentiation time than rat oligodendrocytes. These same results are also observed in secondary oligodendrocyte cultures, although in general late myelin markers such as MBP and PLP are expressed at a much lower level in mouse cells than rat cells.

引用

页码：70 / 80

页数：11