STUDIES WITH A SULFITE OXIDASE-MODIFIED CARBON PASTE ELECTRODE FOR DETECTION DETERMINATION OF SULFITE ION AND SO2(G) IN CONTINUOUS-FLOW SYSTEMS

被引：19

作者：

NADER, PA ^{[1
]}

VIVES, SS ^{[1
]}

MOTTOLA, HA ^{[1
]}

机构：

[1] OKLAHOMA STATE UNIV,DEPT CHEM,STILLWATER,OK 74078

来源：

JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 1990年 / 284卷 / 02期

关键词：

D O I：

10.1016/0022-0728(90)85042-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Modification of carbon paste electrodes by direct admixing of the enzyme sulfite oxidase from chicken liver [EC 1.8.3.1] that was previously immobilized on crosslinked agarose gels activated with 1,1'-carbonyldiimidazole and their amperometric peformance in continuous-flow systems are described. The effect of different experimental parameters in the selective detection/determination of sulfite ion in aqueous solutions and S02(g) in air is reported. Hexacyanoferrate ions, used as redox mediators, cannot be incorporated directly (after electrostatic immobilization) into the electrode but their utilization is possible via two alternatives: (a) directly in solution, or (b) immobilized on poly(4-vinylpyridine) but incorporated as a solid reservoir that "bleeds" the redox mediator slowly into the carrier stream. The second alternative offers some advantages for detection/determination of sulfite ion and SO2(g) at ppb levels. © 1990.

引用

页码：323 / 333

页数：11

共 13 条

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[2]

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[3]

BEUTLER HO, 1985, METHOD ENZYMAT AN, V7, P585

[4] BIOAMPEROMETRIC SENSORS FOR PHENOL BASED ON CARBON PASTE ELECTRODES [J].