CLONING AND CHARACTERIZATION OF THE RAT ALPHA-INHIBIN GENE

被引：17

作者：

ALBISTON, AL ^{[1
]}

LOCK, P ^{[1
]}

BURGER, HG ^{[1
]}

KROZOWSKI, ZS ^{[1
]}

机构：

[1] MONASH UNIV, MED CTR,MED RES CTR,PRINCE HENRYS HOSP CAMPUS, ST KILDA RD, MELBOURNE, VIC 3004, AUSTRALIA

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 1990年 / 68卷 / 2-3期

基金：

英国医学研究理事会;

关键词：

Promoter; S[!sub]1[!/sub] analysis; α-Inhibin gene; rat;

D O I：

10.1016/0303-7207(90)90184-A

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The gene for the rat glycoprotein hormone α-inhibin has been cloned and characterized. The entire gene was found to be contained within a 5.5 kilobase EcoRI fragment. It is composed of two exons separated by a 1.5 kb intron. Primer extension and S1nuclease analysis showed that the major transcription initiation site in the ovary was 77 bp from the start of translation. The promoter region of the gene did not contain a conventional TATA box but instead a number of GA rich repeated sequences were found to be present. Other potential regulatory elements found included a repeating purine-pyrimidine tract (TG)28, cAMP and phorbol ester response elements and a putative glucocorticoid response element. Southern blot analysis of rat genomic DNA indicated that there is a single gene for α-inhibin in the rat. © 1990.

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页码：121 / 128

页数：8

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