REQUIREMENTS FOR THE INACTIVATION OF RIBOSOMES BY GELONIN

被引：28

作者：

SPERTI, S ^{[1
]}

BRIGOTTI, M ^{[1
]}

ZAMBONI, M ^{[1
]}

CARNICELLI, D ^{[1
]}

MONTANARO, L ^{[1
]}

机构：

[1] UNIV BOLOGNA,DIPARTIMENTO PATOL SPERIMENTALE,VIA S GIACOMO 14,I-40126 BOLOGNA,ITALY

来源：

BIOCHEMICAL JOURNAL | 1991年 / 277卷

关键词：

D O I：

10.1042/bj2770281

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Inactivation of Artemia salina and rabbit ribosomes by gelonin requires ATP and a high-M(r) factor present in the rabbit reticulocyte-lysate post-ribosomal supernatant. The kinetic constants of the gelonin-catalysed release of adenine from A. salina ribosomes are K(m) = 4.35-mu-M and K(cat) = 0.1 min-1 in the absence of cofactors, and K(m) = 1.15-mu-M and K(cat) = 108 min-1 in their presence. The last two values are similar to those measured for ricin A chain in the absence of cofactors (K(m) = 2.02-mu-M and K(cat). = 317 min-1).

引用

页码：281 / 284

页数：4

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