CALBINDIN IMMUNOREACTIVITY IN NORMAL HUMAN TEMPORAL NEOCORTEX

被引:51
作者
FERRER, I
TUNON, T
SORIANO, E
DELRIO, A
IRAIZOZ, I
FONSECA, M
GUIONNET, N
机构
[1] HOSP NAVARRA, SERV ANAT PATOL, NAVARRA, SPAIN
[2] UNIV BASQUE COUNTRY, DEPT NEUROCIENCIAS, LEIOA, SPAIN
[3] UNIV NAVARRA, CATEDRA ANAT, PAMPLONA, SPAIN
[4] UNIV BARCELONA, FAC BIOL, UNIDAD BIOL CELULAR, BARCELONA 7, SPAIN
关键词
CEREBRAL CORTEX; LOCAL-CIRCUIT NEURON; CALBINDIN;
D O I
10.1016/0006-8993(92)90447-H
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Calbindin immunoreactivity in the temporal neocortex was examined in 4 subjects with no neurological, metabolic or malignant disease. The brains were obtained between 1 and 4 h after death and rapidly fixed by perfusion with 4% paraformaldehyde through the carotid arteries, cut into slabs, cryoprotected and stored at -80-degrees-C. Sections of the whole left temporal lobe obtained with a freezing microtome were processed free-floating with a well known monoclonal antibody against calbindin according to the peroxidase-antiperoxidase (PAP) method. Calbindin-immunoreactive (CaBP-ir) neurons were found to be local-circuit neurons (interneurons) mainly distributed in the upper cortical layers (layers I, II and III), and were categorized as small multipolar neurons with ascending dendrites ramifying in the molecular layer, small bitufted cells, pyramid-like cells in layer II, horizontal neurons in the molecular layer, multipolar neurons with long descending dendrites, and large double-bouquet cells, some of them exhibiting a very long dendrite with claw-shaped terminals in layer V. Less than 10% of all CaBP-ir neurons were localized in the remaining cortical layers. Pyramidal cells were only very weakly or not stained at all. In addition, CaBP-ir fibres formed a dense plexus in the molecular layer, and vertical bundles 8-10-mu-m thick and 500-600-mu-m long, separated by blank spaces 20-40 microns wide were distributed in layers III and V/VI. CaBP-ir neurons and fibres, through their contacts with pyramidal cells, may regulate contralateral and ipsilateral cortico-cortical connections, may control the vertical neuronal inhibition across different cortical layers, and may regulate the excitability of most pyramidal cells through their terminal bouquets ramifying in the molecular layer.
引用
收藏
页码:33 / 41
页数:9
相关论文
共 56 条
[1]   HIPPOCAMPAL CALCIUM-BINDING PROTEIN DURING COMMISSURAL KINDLING-INDUCED EPILEPTOGENESIS - PROGRESSIVE DECLINE AND EFFECTS OF ANTICONVULSANTS [J].
BAIMBRIDGE, KG ;
MILLER, JJ .
BRAIN RESEARCH, 1984, 324 (01) :85-90
[2]   CALCIUM-BINDING PROTEIN DISTRIBUTION IN THE RAT-BRAIN [J].
BAIMBRIDGE, KG ;
MILLER, JJ ;
PARKES, CO .
BRAIN RESEARCH, 1982, 239 (02) :519-525
[3]   DISTRIBUTION OF PARVALBUMIN IMMUNOREACTIVITY IN THE VISUAL-CORTEX OF OLD-WORLD MONKEYS AND HUMANS [J].
BLUMCKE, I ;
HOF, PR ;
MORRISON, JH ;
CELIO, MR .
JOURNAL OF COMPARATIVE NEUROLOGY, 1990, 301 (03) :417-432
[4]  
Cajal SR, 1911, HISTOLOGIE SYSTEME N
[5]   NUCLEUS BASALIS MEYNERT NEURONS CONTAIN THE VITAMIN-D-INDUCED CALCIUM-BINDING PROTEIN (CALBINDIN-D 28K) [J].
CELIO, MR ;
NORMAN, AW .
ANATOMY AND EMBRYOLOGY, 1985, 173 (02) :143-148
[6]   PARVALBUMIN IN MOST GAMMA-AMINOBUTYRIC-ACID CONTAINING NEURONS OF THE RAT CEREBRAL-CORTEX [J].
CELIO, MR .
SCIENCE, 1986, 231 (4741) :995-997
[7]   CALBINDIN-D-28K AND PARVALBUMIN IN THE RAT NERVOUS-SYSTEM [J].
CELIO, MR .
NEUROSCIENCE, 1990, 35 (02) :375-475
[8]   CALBINDIN IMMUNOREACTIVITY ALTERNATES WITH CYTOCHROME-C-OXIDASE-RICH ZONES IN SOME LAYERS OF THE PRIMATE VISUAL-CORTEX [J].
CELIO, MR ;
SCHARER, L ;
MORRISON, JH ;
NORMAN, AW ;
BLOOM, FE .
NATURE, 1986, 323 (6090) :715-717
[9]   A MICROCOLUMNAR STRUCTURE OF MONKEY CEREBRAL-CORTEX REVEALED BY IMMUNOCYTOCHEMICAL STUDIES OF DOUBLE BOUQUET CELL AXONS [J].
DEFELIPE, J ;
HENDRY, SHC ;
HASHIKAWA, T ;
MOLINARI, M ;
JONES, EG .
NEUROSCIENCE, 1990, 37 (03) :655-673
[10]   VISUALIZATION OF CHANDELIER CELL AXONS BY PARVALBUMIN IMMUNOREACTIVITY IN MONKEY CEREBRAL-CORTEX [J].
DEFELIPE, J ;
HENDRY, SHC ;
JONES, EG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (06) :2093-2097