SIMULTANEOUS DETERMINATION OF ALUMINUM AND BERYLLIUM BY 1ST-DERIVATIVE SYNCHRONOUS SOLID-PHASE SPECTROFLUOROMETRY

A method for the simultaneous determination of aluminium and beryllium in mixtures by first-derivative synchronous solid-phase spectrofluorimetry has been developed. Aluminium and beryllium reacted with morin to give fluorescent complexes, which were fixed on a dextran-type resin. The fluorescence of the resin, packed in a 1-mm silica cell, was measured directly with a solid-surface attachment. The constant wavelength difference chosen to optimize the determination was DELTA-lambda = lambda(em) - lambda(ex) = 75 nm. Aluminium was measured at lambda(ex)/lambda(em) = 445/520 nm and beryllium at lambda(ex)/lambda(em) = 430/505 nm. The range of application is between 0.5 and 5.0 ng/ml for both aluminium and beryllium. The accuracy and precision of the method are reported. The method has been successfully applied to the determination of aluminium and beryllium in synthetic mixtures and natural waters.