TRANSFORMING GROWTH FACTOR-BETA(1) INHIBITS TCDD-INDUCED CYTOCHROME P450IA1 EXPRESSION IN HUMAN LUNG-CANCER A549 CELLS

被引：22

作者：

VOGEL, C ^{[1
]}

DOHR, O ^{[1
]}

ABEL, J ^{[1
]}

机构：

[1] UNIV DUSSELDORF,MED INST ENVIRONM HYG,DEPT TOXICOL,D-40225 DUSSELDORF,GERMANY

来源：

ARCHIVES OF TOXICOLOGY | 1994年 / 68卷 / 05期

关键词：

2,3,7,8-TCDD; TRANSFORMING GROWTH FACTOR-BETA(1); CYTOCHROME P450IA1; LUNG CANCER A549 CELLS;

D O I：

10.1007/s002040050073

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

The effect of transforming growth factor-beta(1) (TGF-beta 1) on the expression of cytochrome P450IA1 (CYPIA1) was examined in 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-treated human lung cancer A549 cells. Using the reverse transcription-polymerase chain reaction (RT-PCR) it was demonstrated that TGF-beta(1) inhibits CYPIA1 expression in a dose dependent manner. Based on the inhibitory concentration 50 (IC50) of about 5 pM it is suggested that TGF-beta(1) has a physiological function in downregulation of this cytochrome. In the presence of cycloheximide, the effect of TGF-beta(1) on CYPIA1 mRNA disappeared. This finding indicates that protein synthesis may be required for the TGF-beta(1) mediated response of CYPIA1. The possible mechanisms by which TGF-beta(1) interacts with TCDD-responsive drug metabolizing enzymes are discussed.

引用

页码：303 / 307

页数：5

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