APPLICATION OF AUTOMATED FLOW-INJECTION ANALYSIS TO DETERMINE NITRITE AND NITRATE IN MOUSE-BRAIN

被引：34

作者：

HABU, H ^{[1
]}

YOKOI, I ^{[1
]}

KABUTO, H ^{[1
]}

MORI, A ^{[1
]}

机构：

[1] OKAYAMA UNIV,SCH MED,INST MOLEC & CELLULAR MED,DEPT NEUROSCI,OKAYAMA 700,JAPAN

来源：

NEUROREPORT | 1994年 / 5卷 / 13期

关键词：

MOUSE BRAIN; NITRIC OXIDE; AUTOMATED FLOW INJECTION ANALYSIS; NITRITE; NITRATE; MICROWAVE IRRADIATION;

D O I：

10.1097/00001756-199408150-00007

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

NITRIC oxide level in the mouse brain was estimated by determination of nitrite and nitrate using an automated flow injection analyser for NOx. Different experimental conditions were examined to determine which produced reproducible results. After pretreatment of tissue specimens by the ZnSO4-NaOH method for deproteinization, reproducible and constant values were obtained. The values were more accurate immediately after sectioning without freezing than after 24 h with freezing. Two sacrifice methods, decapitation and microwave irradiation of the head, were investigated, but there was no significant difference between the two. No substances in the mouse brain exerted a positive or negative influence on the results. These results show that our method is indeed applicable to the brain tissue.

引用

页码：1571 / 1573

页数：3

共 11 条

[1] A CHEMI-LUMINESCENT TECHNIQUE FOR THE DETERMINATION OF NANOMOLAR CONCENTRATIONS OF NITRATE AND NITRITE IN SEA-WATER [J].

GARSIDE, C .

MARINE CHEMISTRY, 1982, 11 (02) :159-167

[2] GLUTAMATE, NITRIC-OXIDE AND CELL CELL SIGNALING IN THE NERVOUS-SYSTEM [J].

GARTHWAITE, J .

TRENDS IN NEUROSCIENCES, 1991, 14 (02) :60-67

[3] REGIONAL STUDIES OF CATECHOLAMINES IN RAT BRAIN .I. DISPOSITION OF [3H]NOREPINEPHRINE [3H]DOPAMINE AND [3H]DOPA IN VARIOUS REGIONS OF BRAIN [J].

GLOWINSKI, J ;

IVERSEN, LL .

JOURNAL OF NEUROCHEMISTRY, 1966, 13 (08) :655-+

[4] ANALYSIS OF NITRATE, NITRITE, AND [N-15]-LABELED NITRATE IN BIOLOGICAL-FLUIDS [J].

GREEN, LC ;

WAGNER, DA ;

GLOGOWSKI, J ;

SKIPPER, PL ;

WISHNOK, JS ;

TANNENBAUM, SR .

ANALYTICAL BIOCHEMISTRY, 1982, 126 (01) :131-138

[5]

HIGUCHI Y, Patent No. 3115974

[6] L-ARGININE INFUSION INDUCES HYPOTENSION AND DIURESIS NATRIURESIS WITH CONCOMITANT INCREASED URINARY-EXCRETION OF NITRITE NITRATE AND CYCLIC-GMP IN HUMANS [J].

KANNO, K ;

HIRATA, Y ;

EMORI, T ;

OHTA, K ;

EGUCHI, S ;

IMAI, T ;

MARUMO, F .

CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY, 1992, 19 (09) :619-625

[7]

Nelson N, 1944, J BIOL CHEM, V153, P375

[8] NITRIC-OXIDE RELEASE ACCOUNTS FOR THE BIOLOGICAL-ACTIVITY OF ENDOTHELIUM-DERIVED RELAXING FACTOR [J].

PALMER, RMJ ;

FERRIGE, AG ;

MONCADA, S .

NATURE, 1987, 327 (6122) :524-526

[9]

Ruzicka J., 1981, FLOW INJECTION ANAL

[10]

SOMOGYI M, 1945, J BIOL CHEM, V160, P69

← 1 2 →