A COMPLEX OF RAB3A, SNAP-25, VAMP/SYNAPTOBREVIN-2 AND SYNTAXINS IN BRAIN PRESYNAPTIC TERMINALS

被引：79

作者：

HORIKAWA, HPM

SAISU, H

ISHIZUKA, T

SEKINE, Y

TSUGITA, A

ODANI, S

ABE, T

机构：

[1] NIIGATA UNIV,BRAIN RES INST,DEPT NEUROCHEM,ASAHIMACHI 1,NIIGATA 951,JAPAN

[2] SCI UNIV TOKYO,INST BIOSCI,287 NODA,JAPAN

[3] NIIGATA UNIV,FAC SCI,DEPT BIOL,NIIGATA 951,JAPAN

来源：

FEBS LETTERS | 1993年 / 330卷 / 02期

关键词：

RAB3A/SMG25A; SYNTAXIN; VAMP/SYNAPTOBREVIN-2; SNAP-25; NEUROTRANSMITTER RELEASE;

D O I：

10.1016/0014-5793(93)80281-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two monoclonal antibodies (SPM-1 and SPM-2) immunoprecipitate brain N-type calcium channels. On immunoaffinity chromatography of digitonin extracts of bovine brain membranes on SPM-1- and SPM-2-Sepharose, proteins of 36 (syntaxins A and B), 28 and 19 kDa are specifically retained by both columns. Here we show that the 19 and 28 kDa bands contain VAMP/synaptobrevin-2, and rab3A/smg25A and SNAP-25, respectively. Since SPM-1 and SPM-2 recognize only syntaxins and the 28 kDa band (rab3A/smg25A and SNAP-25), respectively, the results indicate that all these proteins form a complex. Our results suggest tight linkage between the components involved in neurotransmitter release.

引用

页码：236 / 240

页数：5

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