NEURITOGENESIS IN MOUSE NB2A/D1 NEUROBLASTOMA-CELLS - TRIGGERING BY CALCIUM INFLUX AND INVOLVEMENT OF ACTIN AND TUBULIN DYNAMICS

被引：28

作者：

SHEA, TB ^{[1
]}

机构：

[1] HARVARD UNIV,SCH MED,DEPT BIOL CHEM & MOLEC BIOL,BOSTON,MA 02115

来源：

CELL BIOLOGY INTERNATIONAL REPORTS | 1990年 / 14卷 / 11期

关键词：

actin; Calcium; cytoskeleton; ionophore; neuritogenesis; neuroblastoma; neuronal differentiation; tubulin;

D O I：

10.1016/0309-1651(90)90109-C

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Ionophore (A23187) -mediated calcium influx induced rapid neurite outgrowth in NB2a/d1 cells. This outgrowth was prevented by colchicine but not by cycloheximide, demonstrating a requirement for microtubule assembly but not de novo synthesis. Cytochalasin B induced rapid, colchicine-sensitive outgrowth, indicating that depolymerization of the submembrane actin network may be sufficient to allow neurite outgrowth under conditions which permitted microtubule assembly. Neurites induced by serumdeprivation or calcium influx were rapidly retracted by colchicine unless cytochalasin B was first added, indicating that the actin network may provide the retractile force which mediates neurite retraction following microtubule depolymerization. We conclude that neurite outgrowth can be initiated in NB2a/d1 cells by calcium influx, and may involve alterations in actin and microtubule dynamics. © 1990.

引用

页码：967 / 979

页数：13

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