THE EFFECT OF THE ENDOPHYTE (ACREMONIUM-COENOPHIALUM) AND ASSOCIATED TOXIN(S) OF TALL FESCUE ON SERUM TITER RESPONSE TO IMMUNIZATION AND SPLEEN-CELL FLOW-CYTOMETRY ANALYSIS AND RESPONSE TO MITOGENS

Experiments were conducted with rats and mice to evaluate the effect of the consumption of endophyte (Acremonium coenophialum) and associated toxin (s) infected tall fescue on humoral and cellular aspects of immune function. Treatment diets were: (1) rodent chow (RC) or (2) rodent chow mixed 1 : 1 (w/w) with endophyte infected (E+) or (3) non-infected (E-) tall fescue seed. Rats fed the E+ diet in experiment 1 (43 days) exhibited a lower (P<0.05) serum titer to sheep red blood cell (SRBC) immunization than those fed the E- diet (38.4 vs 131.3). The E+ rats also had lower (P<0.01) white cell counts than either RC or E- groups (5225 vs 8959 and 7491/mm3). Spleen cells from mice fed the E+ diet for 37 days exhibited a reduced (P<0.05) response to the mitogens Concanavalin A and lipopolysaccharide. Flow cytometric analysis revealed a significant (P<0.01) 42% increase in T suppressor cell numbers in spleens of mice fed the E+ vs RC diets. © 1990.