NUCLEAR-MAGNETIC-RESONANCE SPECTROSCOPY TO DETERMINE THE MICELLAR CHOLESTEROL IN HUMAN BILE

被引：30

作者：

ELLUL, JPM

MURPHY, GM

PARKES, HG

SLAPA, RZ

DOWLING, RH

机构：

[1] UNITED MED & DENT SCH GUYS & ST THOMAS HOSP,GUYS HOSP,GASTROENTEROL UNIT,18TH FLOOR GUYS TOWER,LONDON SE1 9RT,ENGLAND

[2] UNIV LONDON,BIRKBECK COLL,DEPT CHEM,LONDON WC1,ENGLAND

来源：

FEBS LETTERS | 1992年 / 300卷 / 01期

关键词：

BILIARY CHOLESTEROL; CHOLESTEROL NMR; MICELLE; VESICLE;

D O I：

10.1016/0014-5793(92)80158-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cholesterol of gallstones comes from the vesicular rather than the micellar phase of bile. Progress in this field has been limited because conventional analytical methods disturb the distribution of cholesterol between the two phases. The resonance of the cholesterol C6 proton occurs at a chemical shift of 5.4 ppm, to be shown by 2D NMR to be specific for biliary cholesterol, and arises only from the micellar mode. Thus integration of the C6 proton resonance peak area provides a direct non-invasive determination of the cholesterol distribution in human bile.

引用

页码：30 / 32

页数：3