EXPRESSION PATTERN OF THE RAR-ALPHA-PML FUSION GENE IN ACUTE PROMYELOCYTIC LEUKEMIA

被引：150

作者：

ALCALAY, M

ZANGRILLI, D

FAGIOLI, M

PANDOLFI, PP

MENCARELLI, A

LOCOCO, F

BIONDI, A

GRIGNANI, F

PELICCI, PG

机构：

[1] UNIV PERUGIA,MONTELUCE POLICLIN,IST CLIN MED 1,I-06100 PERUGIA,ITALY

[2] UNIV ROME 2,CATTEDRA EMATOL,DIPARTIMENTO MED INTERNA,I-00100 ROME,ITALY

[3] UNIV ROME 1,DIPARTIMENTO BIOPATOL,DIV EMATOL,I-00161 ROME,ITALY

[4] UNIV MILAN,OSPED S GERARDO,PEDIAT CLIN,I-20052 MONZA,ITALY

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 11期

关键词：

RECIPROCAL TRANSLOCATION; LEUKEMOGENESIS; TRANSCRIPTION FACTORS;

D O I：

10.1073/pnas.89.11.4840

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Two chimeric genes, PML-RAR-alpha and RAR-alpha-PML, are formed as a consequence of the acute promyelocytic leukemia (APL)-specific reciprocal translocation of chromosomes 15 and 17 [t(15;17)]. PML-RAR-alpha is expressed as a fusion protein. We investigated the organization and expression pattern of the RAR-alpha-PML gene in a series of APL patients representative of the molecular heterogeneity of the t(15;17) and found (i) two types of RAR-alpha-PML mRNA junctions (RAR-alpha exon 2/PML exon 4 or RAR-alpha exon 2/PML exon 7) that maintain the RAR-alpha and PML longest open reading frames aligned and are the result of chromosome 15 breaking at two different sites; and (ii) 10 different RAR-alpha-PML fusion transcripts that differ for the assembly of their PML coding exons. A RAR-alpha-PML transcript was present in most, but not all, APL patients.

引用

页码：4840 / 4844

页数：5

共 17 条

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