SEQUENCE OF AN OLEOSIN CDNA FROM BRASSICA-NAPUS

被引：20

作者：

KEDDIE, JS ^{[1
]}

EDWARDS, EW ^{[1
]}

GIBBONS, T ^{[1
]}

SHAW, CH ^{[1
]}

MURPHY, DJ ^{[1
]}

机构：

[1] UNIV DURHAM,DEPT BIOL SCI,DURHAM DH1 3LE,ENGLAND

来源：

PLANT MOLECULAR BIOLOGY | 1992年 / 19卷 / 06期

关键词：

OLEOSIN; EMBRYOGENESIS; CDNA; BRASSICA-NAPUS; OIL-BODY PROTEIN;

D O I：

10.1007/BF00040541

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Antibodies raised against purified rapeseed 19 kDa oleosin protein were used to screen an embryo-derived lambda-gt11 expression library from Brassica napus. A near full-length cDNA clone, BnV, was isolated. The 781 bp cDNA contained an open reading frame of 549 bp followed by an untranslated region of 222 bp and a poly(A) region of 10 bp. Comparisons between this cDNA and a different oleosin cDNA previously isolated from the same library showed high degrees of sequence similarity in the central domain region and in the 3' untranslated region. Sequence similarities between the derived protein sequence of this cDNA and all other known oleosin protein sequences are discussed.

引用

页码：1079 / 1083

页数：5

共 15 条

[1] LEUCINE ZIPPER MOTIF EXTENDS [J].