AN IMPROVED MTT ASSAY USING THE ELECTRON-COUPLING AGENT MENADIONE

被引：60

作者：

GARN, H ^{[1
]}

KRAUSE, H ^{[1
]}

ENZMANN, V ^{[1
]}

DROSSLER, K ^{[1
]}

机构：

[1] UNIV LEIPZIG,DEPT BIOSCI,D-04103 LEIPZIG,GERMANY

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1994年 / 168卷 / 02期

关键词：

COLORIMETRIC ASSAY; TETRAZOLIUM; MTT; MENADIONE; CYTOTOXICITY ASSAY;

D O I：

10.1016/0022-1759(94)90062-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A modification of the MTT based tetrazolium colorimetric assay is described. Using the electron-coupling agent menadione formazan formation by murine splenocytes and P-815 cells was significantly increased whereas dye reduction by macrophages was hardly influenced. These observations suggest that it should be possible to improve the tetrazolium based cytotoxicity assays of murine macrophages against cells of the syngeneic tumour cell line P-815.

引用

页码：253 / 256

页数：4

共 5 条

[1] USE OF AN AQUEOUS SOLUBLE TETRAZOLIUM FORMAZAN ASSAY TO MEASURE VIABILITY AND PROLIFERATION OF LYMPHOKINE-DEPENDENT CELL-LINES
BUTTKE, TM
MCCUBREY, JA
OWEN, TC
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1993, 157 (1-2) : 233 - 240
[2] MTT COLORIMETRIC ASSAY FOR TESTING MACROPHAGE CYTOTOXIC ACTIVITY INVITRO
FERRARI, M
FORNASIERO, MC
ISETTA, AM
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1990, 131 (02) : 165 - 172
[3] A COLORIMETRIC MICROTITER ASSAY FOR THE QUANTITATION OF CYTOKINE ACTIVITY ON ADHERENT CELLS IN TISSUE-CULTURE
KEISARI, Y
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1992, 146 (02) : 155 - 161
[4] RAPID COLORIMETRIC ASSAY FOR CELLULAR GROWTH AND SURVIVAL - APPLICATION TO PROLIFERATION AND CYTO-TOXICITY ASSAYS
MOSMANN, T
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1983, 65 (1-2) : 55 - 63
[5] AN IMPROVED COLORIMETRIC ASSAY FOR CELL-PROLIFERATION AND VIABILITY UTILIZING THE TETRAZOLIUM SALT XTT
ROEHM, NW
RODGERS, GH
HATFIELD, SM
GLASEBROOK, AL
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1991, 142 (02) : 257 - 265

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