LIVER MICROSOMAL MONOOXYGENASE INDUCTION IN WINTER FLOUNDER (PSEUDOPLEURONECTES-AMERICANUS) FROM A GRADIENT OF SEDIMENT PAH CONCENTRATIONS AT SYDNEY-HARBOR, NOVA-SCOTIA

被引：37

作者：

ADDISON, RF ^{[1
]}

WILLIS, DE ^{[1
]}

ZINCK, ME ^{[1
]}

机构：

[1] FISHERIES & OCEANS CANADA,BEDFORD INST OCEANOG,PHYS & CHEM SCI BRANCH,DARTMOUTH B2Y 4A2,NS,CANADA

来源：

MARINE ENVIRONMENTAL RESEARCH | 1994年 / 37卷 / 03期

关键词：

D O I：

10.1016/0141-1136(94)90055-8

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Winter flounder (Pseudopleuronectes americanus) were sampled in summer 1989 and 1990 in Sydney Harbour, Nova Scotia, along a gradient of sediment polynuclear aromatic hydrocarbon (PAH) concentrations arising from industrial contamination; an uncontaminated bay nearby was used as a reference site. Hepatic mono-oxygenase activity, estimated by measurements of catalytic activity and by immunochemical analysis of cytochrome P-450 1A, increased with sediment PAH concentrations; the extent of induction between the reference and the most contaminated sites ranged up to 50-fold. Cyanoethoxycoumarin O-de-ethylase activity was at least as sensitive an indicator of mono-oxygenase induction as ethoxy-resorufin O-de-ethylase or benzo(a)pyrene hydroxylase, and all three enzyme activities were well correlated with cytochrome P-450 1A concentrations and with sediment PAH concentrations.

引用

页码：283 / 296

页数：14

共 27 条

[1] VARIATION OF HEPATIC ETHOXYCOUMARIN O-DE-ETHYLASE ACTIVITY WITH BODY-WEIGHT AND OTHER FACTORS IN BROOK TROUT (SALVELINUS-FONTINALIS) [J].