IDENTIFICATION OF PHOSPHOCYSTEINE BY ELECTROSPRAY MASS-SPECTROMETRY COMBINED WITH EDMAN DEGRADATION

被引：13

作者：

WEIGT, C ^{[1
]}

KORTE, H ^{[1
]}

POGGE, R ^{[1
]}

VONSTRANDMANN, RP ^{[1
]}

HENGSTENBERG, W ^{[1
]}

MEYER, HE ^{[1
]}

机构：

[1] RUHR UNIV BOCHUM,INST PHYSIOL CHEM 1,BIOCHEM SUPRAMOLEK SYST ABT,PROT SEQUENZLAB,D-44780 BOCHUM,GERMANY

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 712卷 / 01期

关键词：

D O I：

10.1016/0021-9673(95)00457-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Phosphocysteine is an intermediate in the phasphoenolpyruvate-dependent phosphotransferase system (PTS) and in the dephosphorylation of phosphotyrosine residues by protein tyrosine phosphatases. A method is described for the direct and non-radioactive localization of phosphocysteine. The phosphorylated Glu-C peptide of the EII(Mtl) protein of the PTS of Staphylococcus carnosus was identified by LC-electrospray MS and isolated for further analysis. Following chemical modification with alkaline ethanethiol, S-ethylcysteine was identified during Edman degradation, demonstrating that phosphocysteine reacts like phosphoserine. As a control, the unphosphorylated cysteine could not be modified by the alkaline ethanethiol.

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页码：141 / 147

页数：7

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