GEL-PERMEATION CHROMATOGRAPHIC CHARACTERIZATION OF SODIUM HYALURONATE AND ITS FRACTIONS PREPARED BY ULTRASONIC DEGRADATION

被引：25

作者：

CHABRECEK, P

SOLTES, L

KALLAY, Z

NOVAK, I

机构：

[1] SLOVAK ACAD SCI, INST EXPTL PHARMACOL, CS-84216 BRATISLAVA, CZECHOSLOVAKIA

[2] SLOVAK UNIV TECHNOL BRATISLAVA, INST BIOTECHNOL, CS-81243 BRATISLAVA, CZECHOSLOVAKIA

[3] SLOVAK ACAD SCI, INST POLYMER, CS-84236 BRATISLAVA, CZECHOSLOVAKIA

来源：

CHROMATOGRAPHIA | 1990年 / 30卷 / 3-4期

关键词：

Gel permeation chromatography; Hyaluronate fractions; Molecular weight and polydispersity; Ultrasonic degradation;

D O I：

10.1007/BF02274547

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High-molecular-weight sodium hyaluronate isolated from rooster combs was degraded by ultrasonication. The molecular weight of hyaluronate and its polydispersity was determined by gel-permeation chromatography. During 75-min treatment the molecular weight value decreased from 1.39×106 Da to 2.25×105 Da while the polydispersity of the molecular weight increased from 1.29 to 2.36. The reciprocal value of the square of the hyaluronate's molecular weight was linearly proportional to the time of ultrasonication. © 1990 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH.

引用

页码：201 / 204

页数：4

共 20 条

[1] TRANSIENT INTERMEDIATES IN RADIOLYSIS OF HYALURONIC ACID [J].