SEQUENCE OF THE IAA AND IPT REGION OF DIFFERENT AGROBACTERIUM-TUMEFACIENS BIOTYPE-III OCTOPINE STRAINS - RECONSTRUCTION OF OCTOPINE TI PLASMID EVOLUTION

被引:36
作者
PAULUS, F [1 ]
CANADAY, J [1 ]
VINCENT, F [1 ]
BONNARD, G [1 ]
KARES, C [1 ]
OTTEN, L [1 ]
机构
[1] CNRS,INST BIOL MOLEC PLANTES,12 RUE GEN ZIMMER,F-67084 STRASBOURG,FRANCE
关键词
AGROBACTERIUM-TUMEFACIENS; TI PLASMID; EVOLUTION; IS ELEMENTS; HOST RANGE;
D O I
10.1007/BF00023425
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The TA regions of biotype III octopine/cucumopine (OC) Ti plasmids are closely related to the TL region of the biotype I octopine Ti plasmids pTiAch5 and pTi15955. Sequence analysis shows that the limited and wide host range biotype III OC TA regions are derived from a common ancestor structure which lacked the 6a gene found in the biotype I octopine TL region. The TA region of the wide host range OC Ti plasmids has conserved most of the original TL-like structure. In most wide host range OC isolates the TA-iaaH gene is inactivated by the insertion of an IS866 element. However, the TA region of the wide host range isolate Hm1 carries an intact TA-iaaH gene. This gene encodes a biologically active product, as shown by root induction tests and indole-3-acetic acid measurements. The limited host range OC Ti plasmids pTiAB3 and pTiAg57 have shorter TA regions which are derived from a wide host range TA region. The AB3 type arose by an IS868-mediated, internal TA region deletion which removed the iaa genes and part of the ipt gene and left a copy of IS868 at the position of the deleted fragment. The pTiAB3 iaa/ipt deletion was followed by insertion of a second IS element, IS869, immediately 3' of the ipt gene. pTiAg57 underwent the same iaa-ipt deletion as pTiAB3, but lacks the IS868 and IS869 elements. Analysis of the various TA region structures provides a detailed insight into the evolution of the biotype III OC strains.
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收藏
页码:601 / 614
页数:14
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