ISOLATION AND NORTHERN BLOTTING OF RNA FROM ASPERGILLUS-NIDULANS

被引：7

作者：

BRADSHAW, RE

PILLAR, TM

机构：

[1] Leicester Biocentre, University of Leicester, Leicester

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 1992年 / 15卷 / 01期

关键词：

ASPERGILLUS-NIDULANS; NORTHERN BLOTTING; RNA ISOLATION;

D O I：

10.1016/0167-7012(92)90062-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a reliable method for the extraction and Northern blotting of RNA from the filamentous fungus Aspergillus nidulans. The use of glass beads to grind open the fungal mycelium means that samples can be processed very quickly for RNA isolation. A combination of glyoxal/dimethylsulfoxide (Me2SO) RNA denaturation with a thin horizontal agarose/MOPS gel gives consistently high-quality Northern blots.

引用

页码：1 / 5

页数：5

共 7 条

[1] ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE [J].

CHIRGWIN, JM ;

PRZYBYLA, AE ;

MACDONALD, RJ ;

RUTTER, WJ .

BIOCHEMISTRY, 1979, 18 (24) :5294-5299

[2] A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY [J].

FEINBERG, AP ;

VOGELSTEIN, B .

ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) :6-13

[3]

Maniatis T., 1982, MOL CLONING

[4] THE GENETICS OF ASPERGILLUS-NIDULANS [J].

PONTECORVO, G ;

ROPER, JA ;

HEMMONS, LM ;

MACDONALD, KD ;

BUFTON, AWJ .

ADVANCES IN GENETICS INCORPORATING MOLECULAR GENETIC MEDICINE, 1953, 5 :141-238

[5] HSP12, A NEW SMALL HEAT-SHOCK GENE OF SACCHAROMYCES-CEREVISIAE - ANALYSIS OF STRUCTURE, REGULATION AND FUNCTION [J].

PRAEKELT, UM ;

MEACOCK, PA .

MOLECULAR & GENERAL GENETICS, 1990, 223 (01) :97-106

[6] GENETIC-REGULATION OF THE QA GENE-CLUSTER OF NEUROSPORA-CRASSA - INDUCTION OF QA MESSENGER RIBONUCLEIC-ACID AND DEPENDENCY ON QA-1 FUNCTION [J].

REINERT, WR ;

PATEL, VB ;

GILES, NH .

MOLECULAR AND CELLULAR BIOLOGY, 1981, 1 (09) :829-835

[7] HYBRIDIZATION OF DENATURED RNA AND SMALL DNA FRAGMENTS TRANSFERRED TO NITROCELLULOSE [J].

THOMAS, PS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (09) :5201-5205

← 1 →