ISOLATION AND CHARACTERIZATION OF N6-METHYLADENOSINE FROM ESCHERICHIA COLI VALINE TRANSFER RNA

被引：82

作者：

SANEYOSHI, M

HARADA, F

NISHIMURA, S

机构：

[1] National Cancer Center Research Institute, Chuo-ku, Tokyo, Tsukiji

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1969年 / 190卷 / 02期

关键词：

D O I：

10.1016/0005-2787(69)90078-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new minor component has been isolated from Escherichia coli tRNAValI which was identified as 6-methylamino-9-β-d-ribofuranosylpurine (N6-methyladenosine). Precautions were taken against possible conversion of N1-methyladenosine or N6-isopentenyladenosine to N6-methyladenosine during the isolation of the tRNAValI and subsequent liberation of the minor component from the tRNA. The N6-methyladenylic acid obtained by mild ribonuclease treatment of the tRNA was subsequently converted to the corresponding nucleoside and to the base by treatment with E. coli alkaline phosphatase and acid hydrolysis, respectively. The identity of the nucleotide, nucleoside and base thus obtained with corresponding authentic samples was established by comparison of their ultraviolet absorption spectra, paper chromatographic mobilities in several solvent systems, and by low- and high-resolution mass spectra. © 1969.

引用

收藏

页码：264 / +

页数：1

相关论文

共 37 条

[11]

GRIFFIN BE, 1963, BIOCHIM BIOPHYS ACTA, V68, P185

[12] A GENERAL PROCEDURE FOR ISOLATION OF MINOR NUCLEOSIDES FROM RIBONUCLEIC ACID HYDROLYSATES [J].

HALL, RH .

BIOCHEMISTRY, 1965, 4 (04) :661-&

[13] 2-METHYLTHIO N6-(DELTA2-ISOPENTENYL) ADENOSINE - A COMPONENT OF E COLI TYROSINE TRANSFER RNA [J].

HARADA, F ;

GROSS, HJ ;

KIMURA, F ;

CHANG, SH ;

NISHIMURA, S ;

RAJBHANDARY, UL .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1968, 33 (02) :299-+

[14] NUCLEOTIDE SEQUENCE OF OLIGONUCLEOTIDES DERIVED FROM ESCHERICHIA COLI VALINE TRANSFER RNA BY RIBONUCLEASE T1 DIGESTION - COMPARISON OF SEQUENCES NEIGHBORING 3'-TERMINALS AND 5'-TERMINALS AND ANTICODON REGION OF ESCHERICHIA COLI VALINE TRANSFER RNA WITH THOSE OF YEAST VALINE TRANSFER RNA [J].

HARADA, F ;

KIMURA, F ;

NISHIMURA, S .

BIOCHIMICA ET BIOPHYSICA ACTA, 1969, 182 (02) :590-+

[15] CYTOKININS - ISOLATION AND IDENTIFICATION OF 6-(3-METHYL-2-BUTENYLAMINO)-9-BETA-D-RIBOFURANOSYLPURINE (2IPA) FROM YEAST CYSTEINE TRNA [J].

HECHT, SM ;

LEONARD, NJ ;

OCCOLOWITZ, J ;

BURROWS, WJ ;

ARMSTRONG, DJ ;

SKOOG, F ;

BOCK, RM ;

GILLAM, I ;

TENER, GM .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1969, 35 (02) :205-+

[16] LARGE-SCALE PREPARATION OF YEAST SOLUBLE RIBONUCLEIC ACID [J].

HOLLEY, RW .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1963, 10 (02) :186-&

[17] METHYLATED BASES OF TRANSFER RIBONUCLEIC ACID FROM HELA AND L CELLS [J].

IWANAMI, Y ;

BROWN, GM .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1968, 124 (1-3) :472-&

[18] PURINE NUCLEOSIDES .3. METHYLATION STUDIES OF CERTAIN NATURALLY OCCURRING PURINE NUCLEOSIDES [J].

JONES, JW ;

ROBINS, RK .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1963, 85 (02) :193-&

[19] RNA CODONS AND PROTEIN SYNTHESIS .9. SYNONYM CODON RECOGNITION BY MULTIPLE SPECIES FO VALINE- ALANINE- AND METHIONINE-SRNA [J].

KELLOGG, DA ;

DOCTOR, BP ;

LOEBEL, JE ;

NIRENBER.MW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1966, 55 (04) :912-&

[20] NUCLEOTIDE SEQUENCE OF A YEAST TYROSINE TRANSFER RNA [J].

MADISON, JT ;

EVERETT, GA ;

KUNG, H .

SCIENCE, 1966, 153 (3735) :531-&

← 1 2 3 4 →