NOVEL OLIGOSACCHARIDES WITH THE SIALYL-LEA STRUCTURE IN HUMAN-MILK

被引：20

作者：

KITAGAWA, H

NAKADA, H

FUKUI, S

FUNAKOSHI, I

KAWASAKI, T

YAMASHINA, I

TATE, S

INAGAKI, F

机构：

[1] KYOTO UNIV,FAC PHARMACEUT SCI,DEPT BIOL CHEM,SAKYO KU,KYOTO 606,JAPAN

[2] KYOTO SANGYO UNIV,FAC ENGN,DEPT BIOTECHNOL,KITA KU,KYOTO 603,JAPAN

[3] TOKYO METROPOLITAN INST MED SCI,DEPT MOLEC PHYSIOL,BUNKYO KU,TOKYO 113,JAPAN

来源：

BIOCHEMISTRY | 1991年 / 30卷 / 11期

关键词：

D O I：

10.1021/bi00225a020

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated four novel oligosaccharides with the sialyl-Le(a) structure from human milk using a monoclonal antibody, MSW 113. These oligosaccharides were purified by affinity chromatography on a column of the immobilized monoclonal antibody and by high-performance liquid chromatography. The results of structural analyses, i.e., 500-MHz H-1 NMR spectroscopy, fast atom bombardment mass spectrometry, and binding to specific anticarbohydrate antibodies, are consistent with the following structures. [GRAPHICS]

引用

页码：2869 / 2876

页数：8

共 30 条

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